模型信息
中文名称:SARS-CoV Urbani 气管滴鼻联合感染雪貂模型
英文名称:SARS-CoV Urbani intratracheally and intranasally infected ferret model
类型:SARS-CoV感染动物模型
分级:NA
用途:用于SARS-CoV感染研究。
研制单位:美国食品药品管理局@@http://www.fda.gov
保存单位:美国食品药品管理局@@http://www.fda.gov
研究背景
暂无介绍
制备方法
To generate the vaccine, 5.94 mL of virus stock ( 1*10^5 TCID50/mL)was treated with 60 mL of 3.7% formalin for 48 h at 37 °C. The inactivated virus (6 mL) was then dialyzed in 2 L of PBS at room temperature for 48h. Medium from uninfected Vero cells was treated in the same way to prepare mock vaccine.Vaccine was plated on Vero cells for 7 days to confirm inactivation. Vaccination was done intramuscularly at 0.5 mL per leg without adjuvant. The virus challenge ( 1x10^5 TCID50/mL) was split into 2 parts: 0.25 mL was given intratracheally, and 0.25 mL was given intranasally. A laryngoscope was used for the intratracheal portion, which was delivered via a 1-cc syringe with a 22-gauge plastic angiocatheter attached. The intranasal inoculum was delivered to each nostril via a plastic bulb–type pipettor.
评价验证
暂无介绍
生物安全性
暂无介绍
讨论与结论
暂无介绍
了解中析
实验室仪器
合作客户
